Module - LIFE3004 - Advanced Biochemistry Laboratory Work

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Module - LIFE3004 - Advanced Biochemistry Laboratory Work

Programme	SoLS - Life Sciences Modules Database (Level 3) C720 - B.Sc. Hons. Biochemistry and Biological Chemistry (Part 2 - Compulsory) C721 - M.Sci. Hons. Biochemistry and Biological Chemistry (Part 2 - Compulsory)
Year	Select a new weight: https://curriculum.nottingham.ac.uk/2026/view/tree/91656/year_lookup/XXIDXX Year 2026/2027
Accrediting Bodies	https://curriculum.nottingham.ac.uk/2026/view/tree/91656/year_lookup/XXIDXX HEFCE Biochemistry benchmarks Royal Society of Biology RSB Biochemistry subject specific outcomes
Module Code	LIFE3004
Credits	20

Total Credits:

20.00

Level:

Target Students:

All students registered for BSc or MSci degrees in Biochemistry and Biological Chemistry.

Summary Of Content:

This module provides the opportunity to perform a number of fundamental and advanced molecular biology techniques. It will investigate the contribution of individual amino acids to the structure and function of two mitogen-responsive transcription factors.

Procedures to isolate and process DNA samples, carry out the polymerase chain reaction, analyse DNA molecules in the laboratory in conjunction with sequence databases will be used. Proteins will be expressed and purified; their functions will be analysed in biochemical experiments. Individual results and data from the class will be analysed as part of an overall project to investigate relevant scientific questions.

Course Web Links:

Type	Link
Other	http://moodle.nottingham.ac.uk/course/view.php?id=2876

To investigate the influence of mutations and phosphorylation on important transcriptional regulators and how this affects their activity in response to growth factors and signal transduction pathways.

Education Aims:

Offering School:

Life Sciences

Convenor:

Name
Prof Peter Shaw

Semester
Spring UK

Taught Semesters:

Requisites:

Subject	Course Title
LIFEC72406	Practical Gene Cloning

Additional Requirements:

Condition
Only available for students studying Biochemistry & Biological Chemistry

Method and Frequency of Class:

Activity	Number of Weeks	Number of sessions	Duration of a session
Lecture		0.00 week

Please refer to the module timetable online for up to date information on contact hours.

Method of Assessment:

Assessment Type	Weight	Requirements
Dissertation	70.00	Write up of Laboratory Project
Coursework 1	5.00	Questions based on laboratory work
Viva voce	25.00	15 minute oral discussion

Please refer to the module timetable online for up to date information on contact hours.

Assessment Period:

Assessed by end of Spring Semester


	Learning Outcome:
		Experimental skills: carry out PCR and restriction digests, prepare agarose gels and purify DNA molecules, introduce plasmids into prokaryotic cells, prepare plasmid DNA and purify proteins from bacterial cell lysates, perform in vitro protein kinase reactions, use immunological techniques to identify proteins and detect post-translational modifications, assemble and detect DNA:protein complexes, analyse results using digital imaging systems. Transferable skills: use of biological databases and 3D molecular modelling; use of online library and reference management software; oral and written presentation of scientific data.

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