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Learning Activity - Membrane protein Structure and Insertion
Type
ModuleLIFE2077 - Structure, Function and Analysis of Proteins
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MEMBRANE PROTEIN STRUCTURE AND INSERTION - You should understand and be able to explain the expression of membrane proteins in different heterologous systems.
MEMBRANE PROTEIN STRUCTURE AND INSERTION - You should understand how membrane proteins can be solubilised by detergents and non-detergents.
MEMBRANE PROTEIN STRUCTURE AND INSERTION - You should understand how membrane proteins can be purified using affinity or other chromatographic techniques.
MEMBRANE PROTEIN STRUCTURE AND INSERTION - You should understand the principles of reconstitution experiments using high or low protein:lipid ratios for structural or functional work, respectively.
MEMBRANE PROTEIN STRUCTURE AND INSERTION - You should be able to describe example proteins that illustrate principles of membrane insertion, purification and structure.
MEMBRANE PROTEIN STRUCTURE AND INSERTION - You should understand and be able to describe the history of aquaporin structure/function determination.
MEMBRANE PROTEIN STRUCTURE AND INSERTION - You should be able to explain the future use of electron microscopy in membrane protein structural biology as applied to ABC transporters.
MEMBRANE PROTEIN STRUCTURE AND INSERTION - You should understand the limitations of structural biology as applied to membrane proteins.
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