Login
| Type | |
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| Module | LIFE1029 - Genes, Molecules and Cells |
| Programme |  Select a new weight:
           https://curriculum.nottingham.ac.uk/2026/view/tree/91339/course_lookup/XXIDXX
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| Year | https://curriculum.nottingham.ac.uk/2026/view/tree/91339/year_lookup/XXIDXX
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| Duration (mins) |
The quantitation of protein (BSA) and sugar by the Biuret and Nelson's assay. The separation of these two molecules by gel filtration using Sephadex G25
- Outcomes
- Other Activities
- Other Modules
No Group | ||
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Understand the basic concepts spectroscopy and the assays used for determing both protein and sugar |
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Be able to draw and interpret a standard curve |
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Be able to perform simple calculations linking optical density to the mass (and hence concentration) of a substance |
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Understand the principles of gel filtration using Sephadex chromatography and to be able to perform an experiment to separate BSA from glucose, using such a method |
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Be able to calculate, from the OD values, the mass of BSA and glucose that eluted from the column and hence the concentration of these molecules that were applied to the column |
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Be able to draw, using computer software, accurate tables and graphs of the data |
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LIFE2099 - Macromolecules and Macromolecular Assemblies: Structure, Analysis and Advanced Microscopy
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