Understand how DNA is packaged in the nuclei of eukaryotic cells and how this differs from prokaryotic cell

Know the names and roles of the three major eukaryotic RNA polymerases

Be familiar with the organisation of eukaryotic promoters and understand what the different regions do.

Know the difference between specific and general transcription factors and know one model for transcription complex assembly.

Understand the basic principles of RNA splicing, capping and polyadenylation.